This web portal was developed as part of the Leucegene project.


The Surface Protein Annotation Tool (SPAT) is a user-friendly tool that scores proteins according to the chance they have to be located at the cell surface.

In brief

  • SPAT calculates a basic score for each protein based on the combinations of selected (and regularly updated) annotations/terms extracted from multiple public proteomic databases, which is then weighted according to the eventual accumulation of “black-listed” annotation flags.
  • SPAT output also contains a series of public (when available) and in-house annotations including the verified main locations and references to verified antibodies targeting annotated proteins, the pubmed references for protein/term associations, as well as expression data and protein levels in essential human organs and normal haematopoietic progenitors (Leucegene Hemogene expression data), making it a helpful tool for the identification of immunotherapy targets.

See publication for more details

If you used SPAT for your research project, please cite:

Publication pending


JF Spinella, Ph.D. - Sauvageau Lab (

Institute for Research in Immunology and Cancer (IRIC) at University of Montreal, Canada

Last update:

SPAT v3.4 - Jan. 2023

SPAT parameters

Info and density plot